Susceptibility testing of Dientamoeba fragilis ATCC 30948 with iodoquinol, paromomycin, tetracycline, and metronidazole

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Susceptibility testing of Dientamoeba fragilis ATCC 30948 with iodoquinol, paromomycin, tetracycline, and metronidazole.

Susceptibility testing was performed on Dientamoeba fragilis ATCC 30948 in a dixenic culture with Klebsiella pneumoniae and Bacteroides vulgatus. D. fragilis was cocultured with the bacteria in TYGM-9 medium (ATCC medium 1171). The activities of antiparasitic drugs were assessed by counting viable D. fragilis trophozoites with a hemacytometer by trypan blue exclusion. The minimal amebicidal con...

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In vitro susceptibility testing of Dientamoeba fragilis.

Dientamoeba fragilis is a commonly encountered trichomonad which has been implicated as a cause of gastrointestinal disease in humans. Despite the frequency of reports recording infections with this parasite, little research has been undertaken in terms of antimicrobial susceptibility. The aim of this study was to evaluate the susceptibility of D. fragilis to several commonly used antiparasitic...

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Standardized antimicrobial disc susceptibility testing of anaerobic bacteria. I. Susceptibility of Bacteroides fragilis to tetracycline.

A modified Bauer-Kirby-Sherris-Turck method for disc susceptibility testing of anaerobic bacteria is presented. When tetracycline was used against 100 strains of Bacteroides fragilis as a model, reasonably reproducible results were obtained after overnight incubation in both the GasPak atmosphere and an atmosphere achieved by adding 10% CO(2) to a mixture of 10% H(2) and 90% N(2). The minimal i...

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Bacteraemia due to Bacteroides fragilis with reduced susceptibility to metronidazole.

Sir, Surveys of antimicrobial susceptibilities of isolates of the Bacteroides fragilis group showed that isolates were usually susceptible to metronidazole.1 Metronidazole-resistant phenotypes have rarely been reported. Here we report B. fragilis bacteraemia with reduced susceptibility to metronidazole developing during treatment. A 70-year-old man was admitted for excision of rectal adenoma. P...

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Advances on Dientamoeba fragilis Infections

Dientamoeba fragilis is an enteric protozoan parasite that remains neglected, probably due to the misconception that it is uncommon and non-pathogenic. As more information be‐ came available and antimicrobial agents were developed with activity against this para‐ site, it became clear that D. fragilis is responsible of an active infection, associated with symptoms such as abdominal pain and dia...

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ژورنال

عنوان ژورنال: Antimicrobial Agents and Chemotherapy

سال: 1994

ISSN: 0066-4804,1098-6596

DOI: 10.1128/aac.38.5.1157